Wiesneriomyces laurinus (Tassi) P.M. Kirk

Basionym – Volutellaria laurina Tassi

Classification – Wiesneriomycetaceae, Tubeufales, Dothideomycetes, Ascomycota

Index Fungorum number – IF 107371; Facesoffungi number – FoF 09126

Other useful webpages – outlineoffungi.org; fungalgenera.org; Dothideomycetes.org

Description – Saprobic on dead leaves of Dimocarpus longan. Sexual morph: undetermined. Asexual morph: Colonies effuse, yellowish brown. Conidiomata sporodochial, solitary, 3–10 setae arising from the margins of the sporodochial stalk. Setae 140–300 × 3–8 µm (av. 180 × 5 μm, n = 30), subulate, apex acute, septate, thick-walled, deep brown, arising on leaf surface. Conidiophores 30–50 × 2–4 µm (av. 40 × 3 μm, n = 21), emi-mucronematous, close to one another, brown to sub-hyaline at the base, hyaline towards the apex, septate, irregularly branched. Conidiogenous cells 6–9 × 2–4 µm (av. 7×3 μm, n = 26), located at the conidiophores terminal, cylindrical, hyaline, integrated. Conidia 50–70 × 2–4 μm (av. 60×3 μm, n = 30), solitary, clumped together into a semi-mucus, hyaline, smooth, sandy, cylindrical, taper towards both ends that are obtusely rounded, 5–6-septate, prominently constricted at septa, median cells 6–10 × 2–4 µm long, terminal cells 6–10 × 2–4 µm.

Culture characteristics – Colonies on PDA reaching 37–42 mm diam. after 3 weeks, colonies from above: medium dense, fat, slightly raised, rough surface with irregular edges, fluffy not smooth, leaden grey to light grey at the margin, black to olivaceous black in the centre; reverse: grey to light grey at the margin, grey-brown to grey in the centre.

Material examined – China, Guangdong Province, Guangzhou City, Haizhu District, Zhongkai University of Agriculture Engineering, 23° 6′ 32″ N, 113° 16′ 37″ E, alt. 20 m, on the dead leaves of Dimocarpus longan Lour. (Sapindaceae), 23 July 2021, YH. Yang & CF. Liao (ZHKU 22-0008, new host record); living cultures ZHKUCC 22-0008, ZHKUCC 22-0009.

GenBank accession numbers – LSU: OM780294, OM780295; SSU: OM780298, OM780306; ITS: OM780284, OM780286.

Notes – See Jayawardena et al. (2022).

Wiesneriomyces laurinus (ZHKU 22-0008, new host record). a–c Appearance of sporodochia on host. d Squash mount of sporodochium. e, f Conidiophores with setae. g–i Conidia. j Colonies on PDA. Scale bars: d = 100 µm, e, f = 20 µm, g, i = 25 µm.

Phylogram generated from RAxML analysis based on combined LSU, SSU and ITS sequence data for Wiesneriomyces laurinus. The tree is rooted to Bezerromyces brasiliensis (URM7411) and B. pernambucoensis (URM7412). Maximum likelihood bootstrap support values equal to or greater 50% and Bayesian posterior probabilities equal to or greater 0.95 are given above the nodes (ML/BYPP). Ex–type/ ex–epitype strains are in bold. New isolates from South China fungi are in red.