Phyllosticta capitalensis Henn.

Classification – Phyllostictaceae, Botryosphaeriales, Dothideomycetes, Ascomycota

Index Fungorum number – IF168326; Facesoffungi number – FoF 06888

Other useful webpages – outlineoffungi.org; fungalgenera.org; Dothideomycetes.org

Description – Associated with Synsepalum dulcificum leaf spots. Sexual morph: undetermined. Asexual morph: Conidia hyaline, transparent, single-celled, smooth wall, mostly pear-shaped, or long pear-shaped, with a size of 10–15 µm × 5–10 µm (av. 11±0.9 × 6±0.6 µm, n = 50), wrapped by a layer of transparent mucus, with a transparent accessory filament at the distal end of the conidia, 3–15 µm long.

Culture characteristics – Colonies on PDA reaching 4 cm diam. after 7 days at 25 °C, white from above, dark green or dark brown, dark green from below, with raised elevation, margin undulate. Colonies on MEA and OA reaching 3 cm and 2 cm diam. after 7 days at 25 °C, respectively.

Material examined – China, Guangzhou, Guangdong Province, on Synsepalum dulcificum (Schumach. & Thonn.) Daniell., (Sapotaceae), March 2021, Y.H. Huang & L.Q. You, living culture ZHKUCC 21-0107, new host record.

GenBank accession numbers – LSU: OL687398; ITS: OL687395; tef1-α: OL828267; act: OL828268.

Phyllosticta capitalensis (ZHKUCC 21-0107, new host record). a Upper view of colony characteristics on PDA. b Reverse view of colony characteristics on MEA. c Upper view of colony characteristics on OA. d Reverse view of colony characteristics on PDA. e Upper view of colony characteristics on MEA. f Reverse view of colony characteristics on OA. g Hypha. h Conidiogenous cells. i–j Conidia. Scale bars: g–h = 25 µm, i–j = 10 µm.

Maximum likelihood tree inferred from the combined ITS, LSU, gapdh, tef1-α and act sequence data for Phyllosticta capitalensis. The tree is rooted to Botryosphaeria stevensii (CBS 112553). RAxML bootstrap support values and maximum parsimony bootstrap support values equal to or greater than 50% are shown near the nodes (ML/MP). Ex–type/ex–epitype strains are in bold. The new isolate from South China region is indicated in red.