Pestalotiopsis pyrrosiae-linguae H. Li
Classification – Sporocadaceae, Amphisphaeriales, Sordariomycetes, Ascomycota
Index Fungorum number – IF900638; Facesoffungi number – FoF 14691
Other useful webpages – outlineoffungi.org; fungalgenera.org; Sordariomycetes.org; coelomycetes.org
Etymology – referring to the host Pyrrosia lingua, from where it was isolated.
Holotype – MHZU 23-0109
Description – Endophytic on leaves of Pyrrosia lingua. Sexual morph: undetermined. Asexual morph: Conidiomata acervular, saucer-shaped, black, solitary or aggregated in clusters, semi-immersed, superficial, shining, releasing black conidial masses on the surface. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 6–12.5 × 2–3.5 µm (av. 9.4 × 2.4 µm, n = 10), holoblastic, monoblastic, determinate, subcylindrical, hyaline, smooth. Conidia 19–24 × 4–6 µm (av. 21.5 × 5.2 µm, n = 30), fusiform to clavate, straight or slightly curved, 4-septate, slightly or not constricted, darker at the septa, with three brown median cells but not often uniform in colour, and with a hyaline apical cell and a pale brown basal cell, smooth- and thin-walled; basal cell 3.5–6 µm long, funnel-shaped, pale brown, often with a single, filiform, unbranched appendage, 4.5–10(–13) µm long; apical cell 3–5 µm long, conical or sometimes subcylindrical, hyaline, with 2–3 filiform, unbranched apical appendages, 9–25 µm long.
Culture characteristics – Colonies on PDA reaching 65 mm diam. at 25 °C after five days. Colonies filamentous to circular, moderately dense, flat, white, reverse pale yellow. Sparse conidiomata produced in PDA at 25 °C after 42 days, irregular-shaped, scattered, semi-immersed to erumpent, dark brown to black; exuding globose, dark brown to black conidial masses.
Material examined – China, Guangdong Province, Guangzhou City, on leaves of Pyrrosia lingua (Thunb.) Farwell (Polypodiaceae), 16 August 2021, H. Li, LH35-C4028 (MHZU 23-0109, holotype); ex-type culture ZHKUCC 23-0807; ibid., living culture ZHKUCC 23-0808.
GenBank accession numbers – ZHKUCC 23-0807 = ITS: OR199902, tef1-α: OR259260, tub2: OR259258; ZHKUCC 23-0808 = ITS: OR199903, tef1-α: OR259261, tub2: OR259259.
Notes – See Dong et al. (2023)
Pestalotiopsis pyrrosiae-linguae (MHZU 23-0109, holotype). a Front and below view of a five-day-old colony on PDA. b Conidial mass. c–e Conidiophores, conidiogenous cells and conidia. f–n Conidia. Scale bars: c, e–n = 10 μm, d = 20 μm.
Maximum likelihood tree inferred from the combined ITS, tef1-α and tub2 sequence data for Pestalotiopsis camelliae-japonicae, P. pyrrosiae-linguae, and P. zhaoqingensis. Maximum likelihood bootstrap support values equal to or greater than 80% and Bayesian posterior probabilities equal to or greater than 0.95 are given above the nodes (ML/BYPP). The tree is rooted to Neopestalotiopsis magna (MFLUCC 12-0652). Ex-type strains are indicated with “T”. The new species from South China region are indicated in red.
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