Colletotrichum fructicola Prihast., L. Cai & K.D. Hyde
Classification – Glomerellaceae, Glomerellales, Hypocreomycetidae, Sordariomycetes, Ascomycota
Index Fungorum number – IF515409; Facesoffungi number – FoF 06767
Other useful webpages – outlineoffungi.org; fungalgenera.org; Dothideomycetes.org
Description – Associated with leaf spot of Zamia furfuracea. Sexual morph: Ascomata greyish-black, globose to subglobose, with hairs, semi-immersed or completely immersed in PDA. Asci 50–80 × 10–12 μm (av. 60 × 10 μm, n = 30), long, thin-walled, 6–8-spored, clavate or cymbiform. Ascospores 15–20 × 5–7 (av. 16 × 6 μm, n = 50), long, one-celled, with large guttule at the centre, slightly curved to curved with rounded ends. Asexual morph: ceolomycetous, Setaria in acervuli on filter paper, abundant, long, dark brown, smooth-walled, with two or more septa, base truncate, apex sharp, 40–60 × 2–5 μm (av. 60 × 3 μm, n = 10). Conidiophores hyaline, smooth-walled to verruculose, aseptate, unbranched. Conidia 15–20 × 5–6 μm (av. 17 × 5 μm, n = 50), formed from the tip of the conidiophore, one-celled, cylindrical with rounded ends, contents appearing, granular. Appressoria 8–10 × 5–7 μm (av. 10 × 6 μm, n = 10), in slide cultures formed from branched mycelia, terminal, brown to dark brown, near rhombus to irregular.
Culture characteristics – Colonies on PDA, reaching 65 mm diam. after 7 days at 25 ℃, growth rate per day 5 mm/d, grey to green, edge white, regular, reverse dark green, white edge with orange conidial masses.
Material examined – China, Guangdong Province, Shenzhen Botanical Garden, on living Zamia furfuracea L.f. ex Aiton (Zamiaceae), 30 November 2020, J.W. Chen, Z.L. Mai and C. Chen (dried cultures ZHKU 21-0074 and ZHJU 21-0075, new host record), living cultures ZHKUCC 21-0081 and ZHJUCC 21-0082.
Additional material – China, Guangdong Province, Shenzhen Botanical Garden, leaf spot of Bougainvillea sp. (Nyctaginaceae), 30 November 2020, J.W. Chen, Z.L. Mai, C. Chen, (dried cultures ZHKU 21-0076 and ZHJU 21-0077), living cultures ZHKUCC 21-0083 and ZHJUCC 21-0084.
GenBank accession numbers – gapdh: OL672731, OL672732, OL672733, OL672733; act: OL672710, OL672711, OL672712, OL672713; chs-1: OL672721, OL672722, OL672723, OL672724; tub2: OL739538, OL739537, OL739536, OL739535.
Notes – See Manawasinghe et al. (2022).
Colletotrichum fructicola (ZHKUCC 21-0081, new host record). a, b Upper and reverse view on PDA after seven days. c Spore masses. d, e Ascomata. f–i Asci. j, k Ascospores. l, m Conidiogenous cells. n, o Conidia. p, q Setae. r, s Appressoria. Scale bars: f–s = 10 µm.
Maximum likelihood tree inferred from the combined ITS, gapdh, act, chs-1 and tub2 sequence data for Colletotrichum endophyticum, C. fructicola, C. pandanicola, and C. syzygicola. The tree is rooted to C. boninense (CBS123755 and ICMP 18596). Maximum parsimony and maximum likelihood bootstrap support values equal to or greater than 50% are given at the nodes and branches (MP/ML). The new isolates from South China region are indicated in red.