Diaporthe angustiapiculata Senan.

Classification – Diaporthaceae, Diaporthales, Sordariomycetes, Ascomycota

Index Fungorum number – IF900439; Facesoffungi number – FoF 14191

Other useful webpages – outlineoffungi.org; fungalgenera.org; Sordariomycetes.org; coelomycetes.org

Description – Saprobic on petiole of Phoenix roebelenii. Sexual morph: undetermined. Asexual morph: Conidiomata 375–520 × 580–610 µm (av. 500 × 600 µm, n = 10), superficial, scattered on substrate, solitary, compressed subglobose, dark brown, coriaceous, ostiolate, papillate. Conidiomatal wall comprising pale brown, thick-walled, cells of textura angularis. Conidiophores reduce to conidiogenous cells. Alpha conidiogenous cells 19–27 × 4–6 µm (av. 25 × 5 µm), phialidic, terminal, bottle-shaped with narrowed apex, no visible periclinal thickening, straight, aseptate, unbranched, densely aggregated, hyaline, thick-walled. Alpha conidia 6–9 × 3–5 µm (av. 7 × 4.5 µm), lower end round, upper end pointed, aseptate, distinct biguttulate, hyaline. Beta conidia not observed.

Culture characteristics – Colonies on PDA reaching 3 cm diam. after 10 days in dark at 25 °C, floccose, flat, circular, entire margin, white, wooly aerial mycelia, reverse off-white, no pigments produced.

Material examined – China, Guangdong Province, Guangzhou City, Danxia Mountain, on petiole of Phoenix roebelenii O’Brien (Arecaceae), 23 September 2021, I.C. Senanayake, 2R (MHZU 22-0110, holotype), ex-type cultures ZHKUCC 22-0184, ZHKUCC 22-0194.

GenBank accession numbers – ITS: OR164920, OR164921; tef1-α: OR166269, OR166270; tub2: OR166309, OR166310; cal: OR166299, OR166300.

Notes – See Senanayake et al. (2023).

 

Diaporthe angustiapiculata (MHZU 22-0110, holotype). a Conidiomata on substrate. b Cross section of conidioma. c, d Conidia attached to conidiogenous cells. e Surface view of colony on PDA. f Reverse view of colony on PDA. Scale bars: b = 250 µm, c, d = 20 µm

Phylogram generated from maximum likelihood analysis based on combined ITS, tub2, tef1-α and cal sequence data for Diaporthe angustiapiculata, D. arecae, D. campylandrae, and D. longipapillata. Bootstrap support values for maximum likelihood equal to or greater than 50% and Bayesian posterior probabilities from Bayesian inference analysis equal to or greater than 0.90 are given at each node (ML/BYPP). The tree is rooted to Cytospora disciformis (CBS 116827) and C. leucostoma (SXYLt). Ex-type strains are in bold. The new isolates from South China region are indicated in blue.

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