Kirschsteiniothelia guangdongensis Senan. & K.D. Hyde
Classification – Kirschsteiniotheliaceae, Kirschsteiniotheliales, Dothideomycetes, Ascomycota
Index Fungorum number – IF900339; Facesoffungi number – FoF 14167
Other useful webpages – outlineoffungi.org; fungalgenera.org; Dothideomycetes.org
Etymology – based on the region where sample has been collected.
Holotype – MHZU 22-0137
Description – Saprobic on dead branches of unidentified plant. Sexual morph: undetermined. Asexual morph: Colonies on natural substrate, superficial, hairy, black, scattered, single or in groups, with superficial, dark brown to black hyphae. Conidiophores 250–350 μm long, 10–18 μm wide, superficial on host surface, macronematous, mononematous, simple, erect to slightly flexuous, unbranched, septate, not constricted at the septa, dark brown, cylindrical. Conidiogenous cells 15–18 × 9–12 μm (av. 17 × 10 μm, n = 20), monoblastic, integrated, percurrently proliferating, terminal, cylindrical to ampulliform, determinate, dark brown. Conidia 290–300 μm long, 42–50 μm wide at base, 20–22 μm wide at apex, elongated, flask-shaped, straight or slightly curved, rounded at the apex, apical cell paler than others, 13 transverse septa with one longitudinal septum in 5 basal cells, a thin sheath at apex extend up to 3rd cell from the top, blackish brown to black, thin-walled, smooth; secession by schizolysis or rhexolysis.
Culture characteristics – Colonies on PDA reaching 4 cm diam. after 7 days incubated in dark at 25 °C, circular, flat, filiform margin, with moderate aerial mycelia, surface view blackish green with grey aerial mycelia, reserve olivaceous grey.
Material examined – China, Guangdong Province, Guangzhou, Haizhu District, (23°06′28.4″N 113°16′51.7″E), on twigs of unidentified plant, 18 June 2021, Kularathnage N.D., NDK 19–2 (MHZU 22-0137, holotype), ex-type culture ZHKUCC 22-0233.
GenBank accession numbers – ITS: OR164946, LSU: OR164974.
Notes – See Senanayake et al. (2023).
Kirschsteiniothelia guangdongensis (MHZU 22-0137, holotype). a Colonies on natural substrate. b, c Conidiophores with conidia. d, e Conidia. f Surface view of colony. g Reserve view of colony. h–l Conidia of Kirschsteiniothelia tectonae (MFLUCC 12-0050). Scale bars: b–e = 150 μm.
Phylogram generated from maximum likelihood analysis based on combined ITS, LSU and SSU sequence data for Kirschsteiniothelia guangdongensis. Bootstrap support values for maximum likelihood equal to or greater than 50% and Bayesian posterior probabilities from Bayesian inference analysis equal to or greater than 0.90 are given at each node (ML/BYPP). The tree is rooted to Astrosphaeriella bakeriana (MFLUCC 11-0027 and CBS 115556). Ex-type strains are in bold. The new isolate from South China region is indicated in blue.